Etoposide, Thermo Scientific Chemicals

Description

Topoisomerase inhibitor and inducer of apoptosis

Etoposide is used as a inhibits DNA synthesis and is very active against cells in the late S and G2 phases of the cell cycle. Etoposide was reported to be used to serum starve Saos-2 M5(p53-/-) cells which were transfected by electroporation with indicated expression plasmids for flag-E2F1 and myc-EBNA3C for selection of colony forming units, thus studying the blocking effect of EBV Nuclear Antigen 3C on E2F1 Mediated Apoptosis Induced by the DNA Damage Response.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Etoposide is used as a inhibits DNA synthesis and is very active against cells in the late S and G2 phases of the cell cycle. Etoposide was reported to be used to serum starve Saos-2 M5(p53-/-) cells which were transfected by electroporation with indicated expression plasmids for flag-E2F1 and myc-EBNA3C for selection of colony forming units, thus studying the blocking effect of EBV Nuclear Antigen 3C on E2F1 Mediated Apoptosis Induced by the DNA Damage Response.

Solubility
Insoluble in water.

Notes
Store solutions in DMSO at 4°C. For long term storage prepare aliquots and store at -20°C.

Specifications

Chemical Identifiers

• CAS: 33419-42-0
• Molecular Formula: C29H32O13
• Molecular Weight (g/mol): 588.56
• MDL Number: MFCD00869325,MFCD00869325
• InChI Key: VJJPUSNTGOMMGY-MRVIYFEKSA-N
• Synonym: vjjpusntgommgy-nzlmilqcsa
• PubChem CID: 50936917
• IUPAC Name: (10R,11R,15R,16S)-16-{[(2R,4aR,6R,7R,8R,8aS)-7,8-dihydroxy-2-methyl-hexahydro-2H-pyrano[3,2-d][1,3]dioxin-6-yl]oxy}-10-(4-hydroxy-3,5-dimethoxyphenyl)-4,6,13-trioxatetracyclo[7.7.0.0³,⁷.0¹¹,¹⁵]hexadeca-1,3(7),8-trien-12-one
• SMILES: COC1=CC(=CC(OC)=C1O)[C@H]1[C@@H]2[C@H](COC2=O)[C@H](O[C@@H]2O[C@@H]3CO[C@@H](C)O[C@H]3[C@H](O)[C@H]2O)C2=CC3=C(OCO3)C=C12

• Melting Point: 236°C to 251°C
• Color: White
• Quantity: 100 mg
• Merck Index: 14,3886
• Solubility Information: Insoluble in water.
• Optical Rotation: −107° (c=1 in Methanol/Dichloromethane 1:9)
• Formula Weight: 588.56
• Physical Form: Powder
• Chemical Name or Material: Etoposide

Safety and Handling

Hazard Category	H302-H350
Hazard Statement	GHS H Statement H350-H302 May cause cancer. Harmful if swallowed.
Precautionary Statement	P201-P202-P264b-P270-P281-P301+P312-P308+P313-P330-P501c
EINECSNumber	251-509-1
RTECSNumber	KC0190000
TSCA	No
Recommended Storage	Ambient temperatures

SDS

RUO – Research Use Only