Gibco™ ExpiSf™ Protein Production Kit

Gibco™ ExpiSf™ Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells using the ExpiSf Expression System.

Catalog Number	Mfr. No.	Content And Storage
A3767806	Gibco™ A3767806	1000 mL bottle ExpiSf CD Medium, store in the dark at 2°C to 8°C; 4 x 1 mL ExpiSf Enhancer, store (at 2°C to 8°C; Shelf Life: 12 months from date of manufacture)
A3767809	Gibco™ A3767809	6 x 1000 mL bottle ExpiSf CD Medium, store in the dark at 2°C to 8°C; 6 units of 4 x 1 mL ExpiSf Enhancer, store (at 2°C to 8°C; Shelf Life: 12 months from date of manufacture)
A3767807	Gibco™ A3767807	10 L bag ExpiSf CD Medium, store in the dark at 2°C to 8°C; 40 mL ExpiSf Enhancer, store (at 2°C to 8°C; Shelf Life: 12 months from date of manufacture)
A3767808	Gibco™ A3767808	5 x 10 L bag ExpiSf CD Medium, store in the dark at 2°C to 8°C; 5 x 40 mL ExpiSf Enhancer, store (at 2°C to 8°C; Shelf Life: 12 months from date of manufacture)

Description

• Delivers greater than 80% more protein yield in high-density suspension Sf9 cell culture than ExpiSf CD Medium alone
• Includes ExpiSf Enhancer, which is specifically designed to work in concert with ExpiSf CD Medium to significantly boost protein expression from baculovirus-infected insect cell cultures
• Demonstrates direct scalability of baculovirus infection from culture volumes of 4mL in deep-well plates to >3 L in shake flasks

The ExpiSf Protein Production Kit is specifically designed to work with ExpiSf9 Cells (Cat. No. A35243) in the ExpiSf Expression System. The kit supplies sufficient ExpiSf Enhancer and ExpiSf CD Medium to infect 1 L, 10 L, or 50 L of culture. ExpiSf CD Medium is also available for purchase separately (Cat. No. A3767802).

Specifications

• Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
• Format: Liquid
• Expression Mechanism: Cell-Based Expression
• Product Line: ExpiSf™

• Compatible Cells: Insect Cells
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
• Expression System: Baculovirus