Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Catalog Number	Mfr. No.	Quantity
FEREF0651	Thermo Scientific™ EF0651	1000 U
FEREF0652	Thermo Scientific™ EF0652	5 x 1000 U
FEREF0654	Thermo Scientific™ EF0654	300 U

Description

FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.

FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 minutes at 37°C. The enzyme is inactivated in 5 minutes at 75°C . Therefore, removal of alkaline phosphatase is not required prior to ligation.

Highlights

• Recombinant enzyme
• Fast dephosphorylation -10 minutes at 37°C
• Fast and complete inactivation -5 minutes at 75°C
• Simultaneous digestion and dephosphorylation of vector DNA
• 100% active in restriction enzyme and PCR buffers
• One protocol for all types of DNA ends:
  • 5'-overhangs
  • 3'-overhangs
  • blunt-ends
  • single nucleotides
• PCR clean-up in conjunction with Exo I
• Protein dephosphorylation

Specifications

• Concentration: 1 U/μL
• Enzyme: Phosphatase
• Compatible Buffer: PCR Buffer, Enzyme Buffer
• Product Type: Alkaline Phosphatase

For Research Use Only. Not for use in diagnostic procedures.