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Thermo Scientific™ GeneJET RNA Purification Kit PROMO
GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
Supplier: Thermo Scientific™ K0732
Description
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Highlights
- Universal–can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
- Efficient–high yields of total RNA.
- Fast–15 minutes after lysis step
- Pure–an A260/280 ratio > 1.9
- Convenient–spin columns are capped and assembled with collection tubes
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
- RT-PCR
- RT-qPCR
- Northern blotting
- Nuclease protection assay
Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol
Specifications
5 x 600 μL Proteinase K, Store at room temperature until opened, -20°C after opening, 200 mL Lysis Buffer, 200 mL Wash Buffer 1 (concentrated), 100 mL Wash Buffer 2 (concentrated), 125 mL Nuclease-free Water, 250 GeneJET RNA Spin Columns with Collection Tubes, 250 Collection Tubes (2 mL), 250 Collection Tubes (1.5 mL) | |
15 min. (After sample homogenization) | |
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions | |
Not High-throughput Compatible (Manual) | |
250 Preps | |
Room Temperature |
Silica Spin Column | |
50 to 100 μL | |
Total RNA | |
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
Mini | |
Up to 160 μg |
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For Research Use Only. Not for use in diagnostic procedures.