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Thermo Scientific™ GeneJET RNA Purification Kit PROMO

GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

Supplier:  Thermo Scientific™ K0732

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Catalog No. FERK0732PM


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Description

Description

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

  • Universal–can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
  • Efficient–high yields of total RNA.
  • Fast–15 minutes after lysis step
  • Pure–an A260/280 ratio > 1.9
  • Convenient–spin columns are capped and assembled with collection tubes

Applications

Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:

  • RT-PCR
  • RT-qPCR
  • Northern blotting
  • Nuclease protection assay

Includes

Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol

Specifications

Specifications

5 x 600 μL Proteinase K, Store at room temperature until opened, -20°C after opening, 200 mL Lysis Buffer, 200 mL Wash Buffer 1 (concentrated), 100 mL Wash Buffer 2 (concentrated), 125 mL Nuclease-free Water, 250 GeneJET RNA Spin Columns with Collection Tubes, 250 Collection Tubes (2 mL), 250 Collection Tubes (1.5 mL)
15 min. (After sample homogenization)
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Not High-throughput Compatible (Manual)
250 Preps
Room Temperature
Silica Spin Column
50 to 100 μL
Total RNA
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Mini
Up to 160 μg
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