Cytiva HiTrap™ Protein A HP Prepacked Columns

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. Cytiva Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

Catalog Number	Mfr. No.	Quantity	Flow Rate
45-000-052	Cytiva 17040303	5 x 5 mL	<20 mL/min.
45-000-049	Cytiva 17040201	5 x 1 mL	<4 mL/min.
45-000-050	Cytiva 17040203	2 x 1 mL	<4 mL/min.
45-000-051	Cytiva 17040301	1 x 5 mL	<20 mL/min.
45-000-503	Cytiva 29048576	1 x 1 mL	<4 mL/min.

Description

• Prepacked with Protein A Sepharose High Performance media for purification and fractionation of IgG subclasses
• For use with syringe, pump or chromatography system such as KTA design or FPLC System
• Binds 20mg human IgG/mL medium
• Stable over pH 3 to 9 for use and 2 to 10 for cleaning
• Designed for use with a syringe, a peristaltic pump, or a chromatography system
• Maximum pressure: 3 bar
• Column material: polypropylene
• 1 and 5mL columns

Media

• Ligand: Native protein A
• Matrix: 6% cross-linked agarose
• Average particle size: 34μm
• pH stability working range: 3 to 9

1mL columns

• Bed dimensions: 7 x 25mm
• Flow velocity: <4mL/min.

5mL columns

• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.

Specifications

• Binding Capacity: 20 mg/mL IgG Medium
• For Use With (Application): Chromatography System, Manual
• For Use With (Equipment): For syringe, peristaltic pump or a chromatography system
• Content And Storage: 4°C to 8°C, 20% Ethanol