Invitrogen™ pLenti6.2/V5-DEST™ Gateway™ Vector

Lentiviral expression vector for lentiviral-based expression of a target gene in dividing and nondividing mammalian cells

Supplier:  Invitrogen™ V36820

Catalog No. V36820

Includes: pLenti6.2/V5-DEST Gateway Vector Box contains pLenti6.2/V5-DEST, 40μL of 150ng/μL vector in 10mM Tris-HCl, 1mM EDTA, pH 8.0 and pLenti6.2/V5-GW/lacZ control, 20μL of 0.5μg/μL vector in 10mM Tris-HCl, 1mM EDTA, pH 8.0. Stbl3 chemically competent E. coli box contains transformation reagents including 20 single-use 50μL aliquots of Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control.

Description

• The vector has the CMV promoter for driving constitutive expression of the target gene and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker
• Stable expression suited to long-term experiments
• High-throughput screening

Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery

Specifications

• Constitutive or Inducible System: Constitutive
• Promoter: CMV
• Selection Agent (Eukaryotic): Blasticidin
• Selection Marker Promoter: PGK Promoter
• Cloning Method: Gateway
• Vector: pLenti
• For Use With (Application): Viral Expression

• Delivery Type: Lentiviral
• Product Type: Lentiviral Expression Vector
• Content And Storage: pLenti6.2⁄V5-DEST™ Gateway™ Vector Box contains pLenti6.2⁄V5-DEST, 40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0 and pLenti6.2⁄V5-GW⁄lacZ control, 20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0. Store at –20°C.
  
  One Shot™ Stbl3™ chemically competent E. coli box contains transformation reagents including 20 single-use 50 μl aliquots of One Shot™ Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.
• Protein Tag: V5 Epitope Tag
• Quantity: 6 μg
• Product Line: Gateway™, ViraPower™

For Research Use Only. Not for use in diagnostic procedures.