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Invitrogen™ Rhodamine Phalloidin
High-affinity F-actin probe conjugated to red-orange fluorescent dye, tetramethylrhodamine (TRITC)
Supplier: Invitrogen™ R415
Catalog No. R415
Description
Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).
Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and Alexa Fluor conjugates including secondary antibodies.
- Selectively stains F-actin
- Excitiation/Emission: 540/565nm
- Superior to antibody staining
- Optimal for fixed and permeabilized samples
- Very widely cited fluorescent phalloidin conjugate
Cell Analysis, Cell Structure, Cytoskeleton
Order Info
Shipping Condition: Room Temperature
Specifications
| Store in freezer -5°C to -30°C and protect from light. | |
| TRITC (Tetramethylrhodamine Isothiocyanate) | |
| Room Temperature | |
| Phalloidin |
| 540⁄565 | |
| 300 units | |
| Classic Dyes | |
| Actin, Cytoskeleton |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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