Gibco™ Human EGF Recombinant Protein

Human EGF Recombinant Protein

Supplier:  Gibco™ PHG0311L

Catalog No. PHG0311L

Description

Human EGF Recombinant Protein for Western Blot, IHC, ELISA, Ctrl, FN

EGF has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin.

• High purity: no interference from other proteins or contaminants
• High biological activity: more results with less protein
• Proven compatibility: Gibco proteins bioassayed with Gibco media
• High-purity, recombinant, bioactive protein: to help ensure growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein
• High biological activity: in-house activity testing of recombinant proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, anti-viral, and protease assays; specific activity is determined by the dose dependent proliferation of mouse BALB/3T3 cells

Specifications

• Accession Number: NM_001963
• Gene ID (Entrez): EGF
• Purification Method: Sequential Chromatography
• Gene Alias: EGF
• Species: Human
• Content And Storage: Store at ≤ 20°C. Upon initial thawing, we recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. The contents should then be apportioned into working aliquots and stored at ≤ 20°C. Avoid repeated freeze⁄thaw cycles. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
• Endotoxin Level: < 0.1 ng/μg
• Mass: 100 μg
• Shipping Condition: Dry Ice
• Expression System: E. coli
• Protein Form: Recombinant, Ligand
• Protein Subtype: EGF (Epidermal Growth Factor)
• Product Line: Gibco™

• For Use With (Application): Cell Culture
• Molecular Weight (g/mol): 6.2 kDa
• Quantity: 100 ug
• Biological Activity: ED₅₀ range = 0.1-0.4 ng⁄mL, ED₅₀ range = 0.1-0.4 ng⁄mL
• Protein Tag: Untagged
• Classification: Carrier-Free
• Indicator Cell: Balb/3T3 cells
• Reactivity: Human
• Specific Activity: 1.0 x 10⁷ - 2.5 x 10⁶ units⁄mg
• Protein Family: Growth Factors & Receptors
• Form: Liquid
• Research Category: Stem Cell Research, Oncology
• Purity or Quality Grade: 95 %

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.