Protein Biology Methods

Products for Your Workflow

Genes direct the production of proteins that play a significant role in diseases such as cancer, Alzheimer’s, and diabetes. Protein biology helps us understand the output of these genes and the way they influence our health. Through protein isolation, purification, separation, and detection, protein biology provides earlier and more accurate diagnoses.

1. Isolation
2. Purification
3. Separation
4. Detection

Step 1: Isolation

Isolate proteins from your mixture with the products below. This protein biology method will help you start your process and prepare you for future workflow steps. Find protein extraction essentials, dissociation reagents, and cell scrapers, plus additional products and resources.

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Protein Extraction

In vitro Protein Expression Systems

Protease Inhibitors and Phosphatase Inhibitors

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Resources

MilliporeSigma™ BugBuster™ Master Mix Flyer (PDF, 277.5 KB)

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Step 2: Purification

Purification is another necessary protein biology method and involves cell lysis, protein binding, washing, and elution. The protocol used will depend on the protein you’re purifying. To get started, shop prepacked columns, products for protein purification, dialysis and desalting, and more. For further information, download our resources.

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Protein Purification

Dialysis and Desalting

Dialysis Cassettes

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Resources

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Step 3: Separation

Solubility, size, charge, and other protein characteristics are used to separate proteins. These techniques include various forms of chromatography in addition to electrophoresis and dialysis. Find the products you need for each of these methods and more by browsing gels, columns, and reagents. Learn more by downloading our relevant resources.

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Precast Electrophoresis Gels

Ion Exchange Columns

Gel Electrophoresis Reagents and Buffers

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Resources

Thermo Scientific and Invitrogen Protein Gels Welcome Packs Brochure (PDF, 220KB)

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Step 4: Detection

Western blotting and ELISA techniques are frequently used for protein detection. Fluorescence microscopy is also common. Browse related reagents, substrates, and imaging systems to help advance your protein detection protocol. For answers that inform your workflow, download our resources.