Modifying Enzyme Buffers
Modifying Enzyme Buffers
Thermo Scientific™ Buffers for RNA Transctription and Post-Transcriptional Modifications
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
Thermo Scientific™ Agarase (0.5 U/μL)
Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.
Thermo Scientific™ Pyrophosphatase, inorganic (0.1 U/μL)
Catalyze the hydrolysis of inorganic pyrophosphate to two orthophosphates.
Thermo Scientific™ Alkaline Phosphatase Buffer-1 (5X)
For cellular and molecular biology applications
Thermo Scientific™ Kinase buffer II (5X)
For cellular and molecular biology applications
Thermo Scientific™ Kinase buffer I (5X)
For cellular and molecular biology applications
AdipoGen VX-745
Chemical. CAS 209410-46-8. Formula C19H9Cl2F2N3OS. MW 436.3. VX-745 is a potent inhibitor of p38α MAPK IC50=10nM with 20-fold selectivity over p38β and 1000-fold selectivity over closely related kinases, ERK1, MK2 and JNK1-3. p38α is a member of the mitogen-activated protein kinase MAPK family of serine/threonine protein kinases and is widely expressed in endothelial, immune or inflammatory cells and plays a central role in the regulation of proinflammatory cytokine production including TNF-α, IL-1β and IL-6. VX-745 is an anti-inflammatory agent that blocks IL-1β and TNF-α release in whole blood in vitro with IC50 values of 150 and 180nM, respectively. VX-745 showed anti-inflammatory efficacy in an animal model of rheumatoid arthritis and recently anti-neuroinflammatory potential in prevention of early-stage Alzheimers diseases AD.
TARGETMOL CHEMICALS INC PROTEINASE K 500MG
Also available in 100 mg and bulk. Please contact Fisher for quotes. Proteinase K (proteinase K for tritirachium album) is a nonspecific serine protease that is useful for general digestion of proteins. It is active in the presence of SDS or urea and over a wide range of pH (4-12) salt concentrations and temperatures. purity: 98%
Revvity Health Sciences Inc Enzymatic buffer kinase 5X, 10 ml
This buffer is a component of HTRF KinEASE kits developed for an optimal kinase enzymatic step. It can be ordered as a spare part.
Revvity Health Sciences Inc Ultra HiBlock Buffer 5X, 10 mL
Formulation of 5X buffer: 125 mM HEPES, pH 7.4, 0.5% Casein, 5 mg/mL Dextran-500 K, 2.5% Triton X-100, 2.5% Blocking reagent, 2.5% BSA and 0.25% Proclin-300
Revvity Health Sciences Inc Stimulation Buffer 2 (5X), 8 ml
Stimulationreagent optimized and validatedfor use in the IP-One Gq kits.
Revvity Health Sciences Inc Phospho and Total protein blocking reagent, 6 ml
Proteins and phosphoprotein may be prone to degradation following cell lysis, e.g. by endogeneous phosphatases. When running a cell-based assay, an appropriate stabilizing and blocking reagent is thus mandatory in order to prevent any remaining enzymatic activity, as well as to garantee protein integrity and quantification.